Bovine adrenal tyrosine hydroxylase: purification and properties.
نویسندگان
چکیده
منابع مشابه
Studies on tyrosine hydroxylase from bovine adrenal medulla.
Approximately 90% of the total tyrosine hydroxylase activity of bovine adrenal medulla homogenates was localized in the particulate fraction of the homogenate. The particulate enzyme was solubilized by incubation with trypsin, and the solubilized enzyme was partially purified. This enzyme was found to behave like the tyrosine hydroxylase which had been purified from the supernatant fraction by ...
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An enzyme that catalyzes the reduction of Il-dehydrosteroids has been purified approximately 250-fold from acetone powder extracts of bovine adrenal glands. The reaction is specific for DPNH, but no structural requirements of substrate other than the steroid nucleus were evident. Steroids are competitive inhibitors with respect to 21-dehydrocortisol reduction. The reaction catalyzed was shown t...
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متن کاملInhibitors of purified beef adrenal tyrosine hydroxylase.
Two classes of compounds have been investigated as inhibitors of purified beef adrenal tyrosine hydroxylase. Among the aromatic amino acids tyrosine analogues were found to be most potent, particularly those having an a-methyl or 3-halogen substitution. Two normal metabolites, monoand diiodo-tyrosine, were found to bevery effective inhibitors. Inhibition by the amino acid analogues was shown to...
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The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) caused phosphorylation of phosphoproteins of 56-kDa which co-migrated with and had identical pI values to subunits of tyrosine hydroxylase. The phosphorylation was closely correlated with an increase of [3H]3,4-dihydroxyphenylalanine (DOPA) production which is a reflection of increased tyrosine hydroxylase activity. Only those phorbol...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)40366-8